Ascentis® Express OH5 is a high-speed, high-performance liquid chromatography column based on Fused-Core® particle technology. The OH5 phase is a highly polar ligand that possesses 5 hydroxyl groups tethered to the silica via novel proprietary linkage phase chemistry. The phase is designed to provide enhanced HILIC partitioning and limited ion exchange retention. This phase exhibits enhanced retention and performance based on the unique phase chemistry coupled with Fused-Core technology.
Ascentis Express OH5 can be used for basic, acidic, or neutral polar compounds with unique selectivity in HILIC mode, which also allows for improved MS sensitivity due to the higher organic content of mobile phases in this mode of chromatography.
Features
- Exhibits HILIC IEX retention, limited silanol anionic character, and is relatively insensitive to ionic strength
- High column stability
- Column efficiency is equivalent to that observed for columns utilizing sub-2 micron totally porous materials